A COMPARATIVE EVALUATION OF THE SURFACE ROUGHNESS AND ANTIFUNGAL PROPERTY OF VORICONAZOLE, ITRACONAZOLE AND TEA TREE OIL COMBINED WITH SOFT LINER IN INHIBITING THE GROWTH OF CANDIDA ALBICANS -AN IN VITRO STUDY
DOI:
https://doi.org/10.53555/jqcs1659Keywords:
Anti-Fungal Drugs, Surface Roughness, Soft Liner, in vitro study, Candida albicans, VoriconazoleAbstract
Aim
This in vitro study aimed to evaluate and compare the antifungal efficacyand surface roughness, heat-cure acrylic resin with soft liners incorporated with voriconazole, itraconazole and tea tree oil.
Methods and Materials
The antifungal drugs (voriconazole and itraconazole) were crushed using mortar pestle and added to the polymer in a specified ratio (10% w/w). After completing the process, 30 samples each of Permasoft, Permasoft with Voriconazole , Permasoft with Itraconazole and Permasoft with Tea tree oil were made (Fig 3).
The finished samples were stored in distilled water in separate labelled containers at room temperature for 4 weeks. At intervals of 1st, 3rd, 7th ,14th, 21st and 28th day, samples were checked for surface roughness as well as antifungal efficacy.
Preparation for Surface Roughness Assessment
Thin discs of 20 mm diameter * 4mm thickness of Group D, E and F were prepared by using brass metal mould where the brass metal mould was manufactured in IIT Jammu and these were incubated at 37°C and were stored in distilled water. After 1st ,3rd ,7th , 14th, 21st and 28th day, the specimens were checked under Olympus microscope.
Results and Conclusion
Incorporation of Tea tree oil significantly enhanced the antifungal activity against Candida albicans, as indicated by a larger zone of inhibition (p < 0.05). Surface roughness significantly increased in the Soft Liner without incorporation of any antifungal drug , considerably increasing for itraconazole (p < 0.05), whereas tea tree oil caused a significant reduction (p< 0.05).
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