COMPREHENSIVE IN-VITRO ANALYSIS OF ANTIOXIDANT PROPERTIES OF FISETIN USING DPPH, H₂O₂, AND FRAP ASSAYS
DOI:
https://doi.org/10.53555/2azqvz43Keywords:
Fisetin, Antioxidant activity, DPPH assay, Hydrogen peroxide scavenging, FRAP assayAbstract
Background: Oxidative stress is associated with various pathological conditions including cancer, cardiovascular diseases, neurodegeneration, and aging. Natural flavonoids have attracted considerable attention due to their strong antioxidant potential.
Objective: The present study aimed to evaluate the antioxidant activity of fisetin using three different in-vitro methods: DPPH radical scavenging assay, hydrogen peroxide scavenging assay, and ferric reducing antioxidant power (FRAP) assay.
Materials and Methods: Antioxidant activity of fisetin was evaluated using three established in-vitro methods. In the DPPH assay, the ability of fisetin to scavenge free radicals was determined spectrophotometrically at 517 nm. Hydrogen peroxide scavenging activity was measured at 230 nm, while reducing power was evaluated using the FRAP method at 700 nm. Ascorbic acid was used as a standard reference antioxidant.
Results: Fisetin demonstrated significant antioxidant activity in all three assays. In the DPPH assay, fisetin showed concentration-dependent radical scavenging activity with a notable percentage inhibition comparable to the standard antioxidant. The hydrogen peroxide scavenging assay also revealed effective neutralization of reactive oxygen species. In the FRAP assay, fisetin exhibited strong reducing power, indicating its electron-donating ability.
Conclusion: The results indicate that fisetin possesses strong antioxidant potential and may serve as a promising natural antioxidant for pharmaceutical and nutraceutical applications.
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