BIOCHEMICAL STUDY OF THE EFFECT OF ALCOHOLIC EXTRACT OF Catharanthus roseus LEAVES IN SWISS ALBINO MICE

Authors

  • Dr. Preeti Gupta

DOI:

https://doi.org/10.53555/eijmhs.v1i4.264

Keywords:

Catharanthus roseus, leaves, Alcohol extract, pregnancy interruption

Abstract

The effect of the Alcoholic extract of the leaves of Catharanthus roseus was investigated. A dose of 10 mg alcoholic extract was dissolved in 50% alcohol and administered to Swiss albino female mice from day 7 to 9 post-coitum. The 10 mg alcoholic dose proved to be 100% effective in causing pregnancy interruption. The alkaline phosphatase and acid phosphatase enzyme activity of the uterus of the mice significantly decreases after treatment with the alcoholic extract of the leaves of Catharanthus roseus.

Author Biography

Dr. Preeti Gupta

Department of Zoology, LBS Govt College, Kotputli, Jaipur (Rajasthan)

References

 Acharya, Deepak and Srivastava, Anshu (2008): Indigenous Herbal Medicines,Tribal Formulations and Traditional Herbal Practices (Book).

 Badami, S., Aneesh, R., Sarkar, S., Satish Kumar, M.N., Suresh, B., Rajan, S. (2003) Antifertility activity of Derris brevipes variety Coriacea. J. Ethnopharmacology: 84.99 -104. (Paper published in Journal)

 Bitman, J., Cecil, H.C., Mench, M.L. and Wrenn, T.R. (1965): Kinetics of invitro glycogen synthesis in the Oestrogen Stimulate drat Uterus. Endocrinology, 76:63-69.

 Bitman,J.,Cecil,H.C., Wood, J.R., and Wrenn, T.R. (1967): Comparison of oestrogen-induced glycogenesis intheuterus of therat, rabbit and sheep. Acta Endocr., 54., 505-513.

 Bo,W.J., Mara Spin, C.E. and Smith, M.S.(1967):Glycogen synthetase activity in the rat uterus.J. Endocrn.,38: 33-37.

 Bo, W.J., and Atkinson, Co.B.(1952): Histochemical studies of glycogen deposition in the uterus of therat. Anat. Res., 113:91-99

 Bodhankar, S.L., Garg,S.K. and Mathur, V.S., (1974): Antifertility screening of Boettiger, E.G .(1946): Changes in the glycogen and water content of the ratuterus.Cell.,Comp.Physiol.,27,9-14.

 Casey, R.C.D. (1960): Alleged Antifertility plants of India. Ind. J. Med. Sci, 14: 390-600. (Paper published in Journal)

 Cecil,H.C.,Bitman,J and Wrenn,T.R. (1964): Effect of histamine and estrogen on the glycogen content of theratuterus. Endocrinology, 74:701-708.

 Chandhoke,N.,Daftan,P., Gupta, S.C. and Atal, C.K. (1979): Contraceptive activity of Pueraria tuberosa, enhancemen to fits biological activity by trikatu group of drugs (Piplamadi).Inthe 3rd Ann.Conj.Soc., Endocrin.India and Mid Term Conj. Endocrin.Soc. India (Varanasi), p.37.

 Chandhoke,N., Daftan,P., Gupta, S.C. and Atal, C.K. (1979): Contraceptive activity of Pueraria tuberosa, enhancement of its biological activity by trikatu group of drugs (Piplamadi). In the 3rd Ann.Conj.Soc., Endocrin. India and Mid Term Conj. Endocrin.Soc. India (Varanasi), p.37.

 Chauhan,S.,Mathur,R.C.(1992): Catharanthu sroseus as male antifertility agents. Proceedings of Int. Conference on Fertility Regulation, Bombay.

 Chopra, R.N., Chopra,I. C.,Banda, K.L. and Kapoor, L.D.,(1946):Indian. J. Agric.Scie.,16:302: Citedfrom Garg,S.K.,Mathur, V.S.and Choudhary, R.R.(1976): Screeningof Indian plants for antifertility activity. Indian.J. Exp.Biol., 16: 1077-1079.

 Chopra, R.N., Nayar, S.L. and Chopra, 1.C. (1956): Glossary of Indian Medicinal plants CSIR. New Delhi (For book).

 Choudhary. R.R. (1992): Why Herbal medicines? In: Herbal medicine for human health. Regional Publications: SEARO. No. 20. WIIO: pp 1-3. (Paper published in a Book)

 Christie, G.A. (1966): Implantation of the rat embryo: glycogen and alkaline phosphatases. J. Reprod. Fertil, 12:279-294. (Paper published in Journal).

 Cushner. I.M. (1973): The Current status of the use of prostaglandins in induced abortion. Aner.J. Public Health. 63 (3): 189-190. (Paper published in Journal)

 El-SayedandCondell,J. (1981): NaturalProducts,44:289.396.

 Ellinger, J.V.and Kirton, K.J. (1974): Ovum transport in rabbits injected with prostaglandin, EL and F2. Biol. Reprod. 11:93-96(Paper Published in Journal)

 Emmens. C.W. (1970): Postcoital Contraception: British. Med. Bull., 26 (1): 45-51. (Paper published in the journal)female albinorats. Indian. J. Med.Res.,62:831-837.

 Gopal Krishnan, M., and Raja Shekharu Shetty, M.R. (1978): Effect of Papaya (Carica Papaya) on pregnancy and estrus cycle in albino rats of Wistar strain. Indian J. Physiol. Pharmacol., 22;66. (Paper published in Journal)

 Gupta Preeti, Mathur Sadhana, and Joshi, Suresh.Precoital antifertility Activity ofCatharanthus roseus 2010, Int.J.Pharmacol.biol.Sci. 4 (4). 39-44.

 Gupta, P. and Mathur, S (2009); Changes in the uterine phosphatases’ levels in female Swiss albino mice treated with chromatographic fractions of petroleum ether extracts of Vinca rosea leaves, JL. of Herbal Medicine and Toxicology 3 (2)., 143-145. (Paper published in Journal)

 Gupta, Preeti (1994): interruption of Pregnancy after Post-Coital Administration of Certain Substances of Plant origin in Mice. Ph.D. Thesis, University of Rajasthan, Jaipur (Thesis).

 Jacob, D. Sharma, S. and Vyas, D.K. (1986): Estrogen and postcoital antifertility effect of Carica papaya seed in the rat. The Indian Zoologist. 10: 127-129. (Paper Published in Journal).

 Jacob, D., Dhir, R.N.Vyas, D.K.and Bhatt, S. (1988):The possible modeof pregnancy interceptory action of Carica papaya seeds extract in the rat.The Indian Zoologist,12:99- 102.

 Johnson, I.S., Armstrong, J.G., German, M.and Burnett, J. (1963): 23,1390: Cited from Murugan, T., Akbarsha, M. (1991): Antispermatogenic effect of Vinca rosea Linn. Indian. I.Exp. Biol., 29:810-812.

 Kamboj,V.P.(1979): Are view of Indian medicinal plants with interceptive activity. Indian.J.Med.Res.,21:336-355.

 Kanjanopathi, Duangta., Smistri, Yuthana., Panthong Ampai., Taesotikul, Twat., Rattan Panona, Vibron (1981): Post-Coital Antifertility effect of Catharanthus roseus Contraception, 24., 554-567. (Paper published in Journal).

 Kapoor, M., Garg, S.K. and Mathur, V.S. (1974): Anti-ovulatory activity of five indigenous plants in rabbits. Indian. J.Med. Res.,62:1223-1227.

 Karnkov, V.V. and Mats, N.N. (1981):Plants as a potential source of contraceptive drugs.RastitResur., 17:293-299.Cited fromMedicinal and AromaticPlant Abstracts(1982):1 487, 71.

 King, E.J. and Wolten, I.D.P. (1959): Microanalysis in Medical Biochemistry, Churchill, London, 42 pp. (For Book).

 Kirtikar,K.R.andBasu,B.D.(1935):IndianMedicinalPlants-Vol 2,307.

 Lal, G. (1976): Evaluation of Post-Coital antifertility substances and their effects on histochemistry. Ph.D. Thesis University of Rajasthan, Jaipur (Thesis)

 Montgomery, R. (1957): Determination of Glycogen. Arch. Biochem., Biophys., 67:378-386 (Paper published in Journal).

 Murugand,T.,Ruknudin,A.,Thangavelu,R. andAkbarsha,M.A.(1989): Antifertilityeffect of Vinca rosea leafextract on malealbino mice - A sperm parametricstudy - Current Science, 58 (19): 1102-1103.

 Murugand,T.andAkabarsha,M.A.(1991): Anti spermatogenic effect of Vincarosea. IndianJ. Exp.Biol.,29(9):810-812.

 Mutreja. A Agarwal, Meera, Kushwaha, Sonalika, Chauhan, Alka (2008): Effect of Nelumbo mucifera seeds on the reproductive organs of female rats. Iranian. JI of Reprod. Medicine Vol ó, No. I. pp7-11 (Paper published in Journal)

 Nadkarni, A.K. and Nadkarni, K.M. (1954): IndianMateriaMedica. (3rdEdn)., Popular Book Depot.,Bombay.

 Prakash, A.O. and Mathur, A. (1976 b):Screening of Indian Plants for antifertility activity.IndianJ. Exp. Bio'.,14:623

 Sharma,M.M.,Gopal,LalandJacob,D.(1976):Estrogenic and pregnancy interceptor effects of Daucuscarrota Seeds. Indian.J.Exp.Bio.,17:506-508.

 Sharma, Shubra (1989): An Investigation on the fertility of female rats after post-coital administration of seeds of Nigella sativa and Carica papaya. Ph.D. Thesis. University of Rajasthan, Jaipur (Thesis).

 Siziramani,J.A. (1969): Histological aspects of the action of androgens, organs and estrogens.An. Internl.Symp. Zeyden., Sponsored by CIBA, Berlin Stanley, Abrahamand Akbarsha, M.A.(1992): Vincristine can cause giant cell formation in rat testis. CurrentSci., 62 (7): 11.

 Vasudeva Neeru and Sharma, S.K. (2007): Post-coital Antifertility activity of Hibiscus rosa sinensis Linn. Roots. Evidence. Based. Compl. And Alt. Med.., Vol. S No l, pp. 91-94 (Paper Published in Journal).

 Vasudeva, N. and Sharma, S.K. (2006). Post coital Antifertility effect of Achyranthes aspera Linn. roots. J. Ethnopharmacology, 107: 179-81. (Paper published in Journal).

 Walaas, O. (1952): Effect of Estrogen on the glycogen content of the rat uterus. Acta. Endoerm., 10: 175-192. (Paper Published in Journal)

 Yadav, R. and Jain, G.C. (1992): Antifertility effect of the aqueous extract of the seeds of Cassia fistula in female rats. Adv. in Contraception, 15:293-301. (Paper Published in Journal)

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Published

2015-12-12