ANTIBACTERIAL EFFECT OF PROPOLIS MODIFIED AND NON PROPOLIS MODIFIED COLLAGEN MEMBRANE AGAINST P.GINGIVALIS AND S. MUTANS – AN IN VITRO STUDY
DOI:
https://doi.org/10.69980/4vjdp173Keywords:
Guided tissue regeneration, Biomaterial modification, Oral biofilm, Ethanolic extract of propolis, Colony-forming units (CFU)Abstract
Background: Collagen Membranes used in Guided tissue regeneration, are prone to microbial colonization, which can reduce their therapeutic efficiency. Propolis, a naturally occurring antibacterial agent, may be added to enhance the clinical results and strengthen antimicrobial qualities.
Aim: To evaluate and compare the antibacterial efficacy of propolis modified and non propolis modified collagen membrane against Streptococcus mutans and Porphyromonas gingivalis
Materials & Methods: An in vitro comparative study was conducted using sterile collagen membrane discs. An ethanolic extract of propolis (EEP) was added to test membranes. The agar diffusion assay (zone of inhibition) and colony forming unit (CFU) measurement were used to evaluate the antibacterial efficacy. Propolis extract alone serves as the reference control
Results: Propolis extract alone demonstrated the highest antibacterial activity. Unmodified collagen membranes exhibited negligible inhibition. Propolis-modified membranes showed measurable antibacterial effects against both microorganisms. Zones of inhibition were more pronounced against P. gingivalis. CFU analysis revealed substantial bacterial reduction with propolis treatment, with the modified membranes demonstrating intermediate efficacy compared to propolis extract alone. These differences were statistically significant (P <0.05)
Conclusion: propolis incorporation strengthen the antibacterial capability of collagen membranes, and may increase their performance during periodontal regeneration
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