ASSESSMENT OF BACTERIAL ADHERENCE AND BIOFILM FORMATION OF STREPTOCOCCUS MUTANS AND PORPHYROMONAS GINGIVALIS ON XENOGRAFT AND PERIOGLAS BONE GRAFT MATERIALS – AN IN VITRO STUDY
DOI:
https://doi.org/10.69980/g6wwey90Keywords:
Biofilm formation, Bacterial adherence, Xenograft, PerioGlasAbstract
Background
Bacterial colonization and biofilm formation on bone graft materials can compromise periodontal regenerative outcomes. Xenografts and bioactive glass (PerioGlas®) are widely used graft materials; however, limited data exist regarding their susceptibility to microbial adherence.
Aim
To evaluate and compare the bacterial adherence and biofilm formation of Streptococcus mutans and Porphyromonas gingivalis on xenograft and PerioGlas® bone graft materials under in vitro conditions.
Materials And Methods
An in vitro study was conducted using xenograft and PerioGlas® samples inoculated with S. mutans and P. gingivalis. After incubation, biofilm formation was assessed using crystal violet staining. The intensity of staining was evaluated to quantify bacterial adherence and biofilm formation. All experiments were performed in triplicate.
Results
PerioGlas® demonstrated significantly reduced biofilm formation compared to xenograft. The percentage of biofilm inhibition by PerioGlas® was 19.64% for S. mutans and 36.1% for P. gingivalis, indicating superior resistance to bacterial colonization. P-values <0.05 were considered statistically significant.
Conclusion
PerioGlas® exhibited significantly lower bacterial adherence and biofilm formation than xenograft. These findings suggest that PerioGlas® may offer improved resistance to microbial colonization, potentially enhancing the success and predictability of periodontal regenerative procedures.
References
1.Allan, I., Newman MG, H. and Wilson, M. (2001) ‘Antibacterial activity of particulate bioglass against supra- and subgingival bacteria’, Journal of Dentistry, 29(2), pp. 107–112.
2.Busscher, H.J. and Van der Mei, H.C. (2006) ‘How do bacteria know they are on a surface and regulate their response to an adhering state?’, Trends in Microbiology, 14(10), pp. 442–448.
3.Chen, W.A. and Dou, Y. (2023) ‘Local and systemic effects of Porphyromonas gingivalis infection’, Microorganisms, 11(2), p. 423.
4.Cortellini, P. and Tonetti, M.S. (2015) ‘Clinical concepts for regenerative therapy in intrabony defects’, Journal of Periodontology, 86(2 Suppl), pp. S1–S6.
5.Di Giulio, M., Traini, T., Sinjari, B., Caputi, S. and Nostro, A. (2016) ‘Porphyromonas gingivalis biofilm formation on different titanium surfaces’, Journal of Biological Regulators and Homeostatic Agents, 30(2), pp. 325–332.
6.Ferraz, M.P., Monteiro, F.J. and Manuel, C.M. (2023) ‘Bone grafts in dental medicine: an overview of autografts, allografts and synthetic materials’, Materials, 16(11), p. 3842.
7.Lasserre, J.F., Brecx, M.C. and Toma, S. (2018) ‘Oral microbes, biofilms and their role in periodontal and peri-implant diseases’, Dentistry Journal, 6(4), p. 49.
8.Meza-Siccha, A.S. and Aguilar-Luis, M.A. (2019) ‘In vitro evaluation of bacterial adhesion and viability of Streptococcus mutans, Streptococcus sanguinis and Porphyromonas gingivalis on titanium and zirconium dental implant abutments’, International Journal of Dentistry, 2019, Article ID 3578701.
9.Nazir, M.A. (2017) ‘Prevalence of periodontal disease, its association with systemic diseases and prevention’, International Journal of Health Sciences, 11(2), pp. 72–80.
10.Newman MG., Takei, H., Klokkevold, P.R. and Carranza, F.A. (2021) Newman and Carranza’s Clinical Periodontology. 13th edn. Philadelphia: Elsevier.
11.Schallhorn, R.A. (2014) ‘Periodontal regeneration: management of periodontal osseous defects’, Compendium of Continuing Education in Dentistry, 35(5), pp. 321–329.
12.Stoor, P., Söderling, E., Salonen, J.I. and Konttinen, Y.T. (1998) ‘Antibacterial effects of a bioactive glass paste on oral microorganisms’, Acta Odontologica Scandinavica, 56(3), pp. 161–165.
13.Wang, H.L., Yuan, K., Burgett, F., Shyr, Y. and Syed, S. (1994) ‘Adherence of oral microorganisms to guided tissue regeneration membranes’, Journal of Periodontology, 65(3), pp. 211–218.
14.Zhao, R., Yang, R., Cooper, P.R., Khurshid, Z. and Shavandi, A. (2021) ‘Bone grafts and substitutes in dentistry: a review of current trends and developments’, Bioengineering, 8(3), p. 46.
15.Allan, I., Wilson, M. and Newman, H. (2001) ‘Influence of bioactive glass on oral biofilm formation’, Clinical Oral Investigations, 5(1), pp. 41–46
